Ukrainian Antarctic journal

No 14 (2015): Ukrainian Antarctic Journal
Articles

The optimization of chromatographic conditions for testing of Antarctic region hydrobionts tissue extracts

D.V. Gladun
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
T.B. Vovk
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
N.G. Raksha
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
O.M. Savchuk
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
L.I. Ostapchenko
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
Published January 12, 2016
Keywords
  • Antarctic marine hydrobionts,
  • gel filtration chromatography,
  • enzyme-electrophoresis

Abstract

Conditions for providing optimal fractionation of tissue extracts bу gel filtration chromatography were selected. Further enzyme-electrophoretic analysis of the obtained protein fractions revealed the presence in extract of Actinaria and Sterechinus neumayer the active forms of the enzymes.