Ukrainian Antarctic journal

No 10-11 (2012): Ukrainian Antarctic Journal
Articles

Introduction in culture in vitro of Deschampsia аntarctica Desv. (Poaceae) from two regions of Maritime Antarctica

O. M. Zahrychuk
Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil
N. M. Drobyk
Ternopil Volodymyr Hnatiuk National Pedagogical University, Ternopil
I. A. Kozeretska
Taras Shevchenko National University of Kyiv, Kyiv
I. Yu. Parnikoza
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
V. A. Kunakh
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
Published December 31, 2012
Keywords
  • Deschampsia antarctica Desv.,
  • seeds germination,
  • micropropagation,
  • callus formation,
  • tissue culture

Abstract

There were developed conditions for seeds germination, micropropagation as well as for induction of callus formation from root and stem explants for a long-term Deschampsia antarctica Desv. tissue culture growing. Optimal for micropropagation of the species was nutrient medium B5 with 0,2 mg/l Kin; for callusogenesis of both explants types – the same medium supplemented with 0,9 mg/l 2,4-D and 0,09 mg/l BAP. Callusogenesis from the root explants considerably exceeded (2-2,6 times) that from the stem one.