Ukrainian Antarctic Journal

No 3 (2005): Ukrainian Antarctic Journal
Articles

Interpopulation heterogeneity of Deschampsia antarctica Desv. according to the variability of nuclei areas and the relative level of DNA in different tissues of leaves

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  • I. Parnikoza,
  • N. Myryuta,
  • M. Mazur,
  • D. Maidanyuk,
  • I. Kozeretska
I. Parnikoza
Institute of molecular biology and genetics of NAS of Ukraine, Kyiv
N. Myryuta
Institute of molecular biology and genetics of NAS of Ukraine, Kyiv
M. Mazur
Taras Shevchenko National University of Kyiv, Kyiv
D. Maidanyuk
Institute of molecular biology and genetics of NAS of Ukraine, Kyiv
I. Kozeretska
Taras Shevchenko National University of Kyiv, Kyiv
DOI: https://doi.org/10.33275/1727-7485.3.2005.578
Published December 15, 2005
Keywords
  • population variability,
  • Deschampsia antarctica,
  • Antarctica
How to Cite
Parnikoza, I., Myryuta, N., Mazur, M., Maidanyuk, D., & Kozeretska, I. (2005). Interpopulation heterogeneity of Deschampsia antarctica Desv. according to the variability of nuclei areas and the relative level of DNA in different tissues of leaves. Ukrainian Antarctic Journal, (3), 128-134. https://doi.org/10.33275/1727-7485.3.2005.578

Abstract

There are few higher plants inhabiting ice-free land in Antarctica: Deschampsia antarctica Desv., Colobanthus quitensis (Kunth.) Bartl., Poa annua L. Research of genetic structure of D. antarctica populations revealed low heterogeneity. The objective of the present work was to analyze diversity in several sub-Antarctic populations studying the morphological variability of the average nucleus area in the D. antarctica leaf epidermis and palisade parenchyma and to assess the ability for polysomaty in these tissues. The investigated material gathered on 5 islands of sub-Antarctica: Petermann, Berthelot, Galindez, Rasmussen and Yalour, thus the populations experienced quit different conditions. The samples were preliminarily fixed during 30 minutes in the acetous alcohol and then colored according to Feulgen (4 preparations for each locality). Then 25 nuclei from each preparation were photographed using a digital camera and the microscope optical system. The explored nucleus area and its coloration intensity was compared with that of colored anaphase cells of D. antarctica rootlet (where the quantity of DNA was assumed to be 4С) using Corel Draw 7.0 software package (including Photo Paint 7.0 and Scion Image). Distribution curves for both traits were constructed for each locality. To compare the corresponding distributions of different localities we used the median criterion method. The investigation of the relative DNA level proved the polysomaty processes in the parenchyma and epidermis of D. antarctica leaves in every locality. The interpopulation heterogeneity was revealed of average nucleus area and relative DNA level in both tissues studied. The result probably arises from the lability of polyploidization adaptive system and its individual character in the Antarctic conditions.

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