The prospects of extraction of collagenolytic enzymes from hydrobionts of Antarctic region
- marine hydrobionts,
- protein fractions,
- enzyme-electrophoresis,
- collagenolytic and trypsin-like activities
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Abstract
Marine organisms have been recognized as rich sources of bioactive compounds with valuabe biotechnology potential. Enzymes extracted from marine hydrobionts have gained much attention because of their unique quite specific properties that determined their profound applications in chemical, medical, food industries and molecular biology experiments. In this regard, our work focused оn investigation of proteolytic potential of marine hydrobionts. At first, tissue extract was separated bу gel filtration chromatography. Further zymography of obtained fractions revealed the presence of active enzymes which further were identified as collagenolytic.
References
- Bradford, M.M. (1976). A rapid and sensitive method for quantities of utilizing the principle of protein binding. Analytical Biochemistry, 86, 193-200.
- Flood, J., Mayne, J., & Robinson, J.J. (2000). Identification and characterization of gelatin-cleavage activities in the apically located extracellular matrix of the sea urchin embryo. Biochemistry and Cell Biology, 78, 455-462.
- Fuchise, T., Sekizaki, H., Kishimura, H., Klomklao, S., Nalinanon, S., Benjakul, S., & Chun, B. (2014). Simple preparation of pacific cod trypsin for enzymatic peptide synthesis. Journal of Amino Acids, 8, 1-8.
- Ghamari, M., Hosseininaveh, V., Darvishzadeh, A., & Talebi, K. (2014). Biochemical characterization of the tissue degrading enzyme, collagenase, in the spined soldier bug, Podisus maculiventris (Hemiptera: Pentatomidae). Journal of Plant Protection Research, 54, 164-170.
- Kim, S.K., Park, P.J., Kim, J.B., & Shahidi, F. (2002). Purification and characterization of a collagenolytic protease from the Filefish, Novoden modestrus. Journal of Biochemistry and Molecular Biology, 35(2), 165-171.
- Moore, S., & Stein, W. (1954). A modified ninhydrin reagent for the determination of amino acids and related compounds. Journal of Biological Chemistry, 211, 907-913.
- Ostapchenko, L., Savchuk, O., & Burlova-Vasilieva, N. Enzyme electrophoresis method in analysis of active components of hemostasis system. Advances in Bioscience and Biotechnology, 2, 20-26.
- Peck, L.S., Webb, K.E., & Bailey, D.M. (2004). Extreme sensitivity of biological function to temperature in Antarctic marine species. Functional Ecology, 18, 625-630.
- Ramundo, J., & Gray, M. (2009). Collagenase for enzymatic debridement: a systematic review. Journal of Wound Ostomy and Continence Nursing, 36(6), 4-11.
- Rudenskaya, G.N., Kislitsin, Yu.A., & Rebrikov, D.V. (2004). Collagenolytic serine protease PC and trypsin PC from king crab Paralithodes camtschaticus: cDNA cloning and primary structure of the enzymes. BMC Structural Biology, 4, 1-9.
- Salamonea, M., Cuttittab, A., Seiditac, G., Mazzolad, S., Bertuzzie, F., Ricordif, C., & Ghersig, G. (2012). Characterization of collagenolytic/proteolytic marine enzymes. Chemical Engineering Transactions, 27, 1-6.
- Songklanakarin, S.K. (2008). Digestive proteinases from marine organisms and their applications. Journal of Science & Technology, (30)1, 37-46.
- Wilkesman, J., & Kurz, L. (2009). Protease analysis by zymography: a review on techniques and patents. Recent Patents in Biotechnology, 3, 175-184.
- Xavier, L.P., Almeida Oliveira, M.G., Guedes, R.N.C., Santos, A.V., & De Simone, S.G. (2005). Trypsin-like activity of membrane-bound mitgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae). European Journal of Enthomology, 102, 147-153.