Ukrainian Antarctic journal

No 14 (2015): Ukrainian Antarctic Journal
Articles

The prospects of extraction of collagenolytic enzymes from hydrobionts of Antarctic region

N.G. Raksha
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
D.V. Gladun
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
O.M. Savchuk
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
L.I. Ostapchenko
ESC “Institute of Biology” of Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601, Ukraine
Published January 12, 2016
Keywords
  • marine hydrobionts,
  • protein fractions,
  • enzyme-electrophoresis,
  • collagenolytic and trypsin-like activities

Abstract

Marine organisms have been recognized as rich sources of bioactive compounds with valuabe biotechnology potential. Enzymes extracted from marine hydrobionts have gained much attention because of their unique  quite specific properties that determined their profound applications in chemical, medical, food industries and molecular biology experiments. In this regard, our work focused оn investigation of proteolytic potential of marine hydrobionts. At first, tissue extract was separated bу gel filtration chromatography. Further zymography of obtained fractions revealed the presence of active enzymes which further were identified as collagenolytic.