Ukrainian Antarctic Journal

No 10-11 (2012): Ukrainian Antarctic Journal
Articles

Geographical gradient of genetic diversity of Deschampsіa antarctіca Desv. from the Maritime Antarctic

I. O. Andreev
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
R. A. Volkov
Yuriy Fedkovych Chernivtsi National University, Chernivtsi
I. A. Kozeretska
Taras Shevchenko National University of Kyiv, Kyiv
I.Yu. Parnikoza
Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv
K. V. Spiridonova
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
S. S. Kiryachenko
Taras Shevchenko National University of Kyiv, Kyiv
D. N. Maydanyuk
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv; Luhansk Taras Shevchenko National University, Luhansk
Bio
V. A. Kunakh
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
Published December 31, 2012
Keywords
  • Deschampsіa antarctіca,
  • RAPD-analysis,
  • ITS rDNA,
  • Maritime Antarctic
How to Cite
Andreev, I. O., Volkov, R. A., Kozeretska, I. A., Parnikoza, I., Spiridonova, K. V., Kiryachenko, S. S., Maydanyuk, D. N., & Kunakh, V. A. (2012). Geographical gradient of genetic diversity of Deschampsіa antarctіca Desv. from the Maritime Antarctic. Ukrainian Antarctic Journal, (10-11), 282-288. https://doi.org/10.33275/1727-7485.10-11.2012.308

Abstract

In order to study the genetic diversity of D. antarctіca Desv. and phylogenetic relationships between the plants from Antarctica and outside of its borders RAPD-analysis and comparative sequence analysis of rDNA internal transcribed spacers 1 and 2 region (ITS1-2) were conducted. There are 15 samples were studied from two distant regions, namely from King George Island and from islands of Argentine archipelago region. The results of RAPD-analysis indicate the differentiation of the studied populations at the molecular genetic level and the existence of a geographical gradient of genetic diversity of D. antarctіca in Maritime Antarctica with a decrease in this parameter from north to south. Sequence analysis of the ITS1-2 rDNA region revealed the presence of several variants in Antarctic populations that differ by specific mutations from plants growing outside Antarctica.

References

  1. Parnikoza, I., Smykla, Ye., Kozeretska, I. et al. (2009). Osoblyvosti antarktychnoi travianystoi tundry v umovakh dvokh riznykh ekolohichnykh hradiientiv [Specifics of Antarctic herbaceous tundra in the conditions of two different ecological gradients]. Visnyk Ukr. tov-va henetykiv i selektsioneriv, 7(2), 218-226.
  2. Parnikoza, I., Ivanets, V., Dykyi, I. et al. (2011). Dominikanskyi martyn yak potentsiinyi perenosnyk kliuchovykh komponentiv nazemnykh ekosystem u pryberezhnii Antarktytsi [Larus dominicanus as a potential carrier of key components of terrestrial ecosystems in the maritime Antarctic]. Antarktyka i hlobalni systemy Zemli: novi vyklyky ta perspektyvy. V Mizhn. Antarktychna konf. (Kyiv, Ukraine, 17-19 May 2011). Kyiv, 2011. - pp. 232-233.
  3. Spiridonova, E., Adnof, D., Andreev, I. et al. (2007). Stabil`nost` genoma vy`sokoproduktivnoj kletochnoj linii K-27 Rauwolfia serpentina Benth. pri izmenenii uslovij vy`rashhivaniya [Stability of the genome of the highly productive Rauwolfia serpentina cell line K-27 when changing culture conditions]. Biopolymers and Cells, 23(2), 86-92.
  4. Kozeretska, I., Parnikoza, I., Mustafa, O. et al. (2010). Development of Antarctic herb tundra vegetation near Arctowski station, King George Island. Polar Science, 3, 254-261.
  5. Marsz, A. (2001). The origin and classification of ice free areas ("oases") in the region of the Admiralty Bay (King George Island, The South Shetland Islands, West Antarctica). In P. Prosek et al. (Eds.), Ecology of the Antarctic Coastal Oasis. Brno, Masaryk University, 7-18.
  6. Parnikoza, I., Kozeretska, I., & Kunakh, V. (2011). Vascular plants of the Maritime Antarctic: Origin and adaptation. American Journal of Plant Science, 2, 381-395.
  7. Parnikoza, I., Miryuta, N., Maidanyuk, D. et al. (2007). Habitat and leaf cytogenetic characteristics of Deschampsia antarctica Desv. in the Maritime Antarctica. Polar Science, 1, 121-128.
  8. Schluter, P., & Harris, S. (2006). Analysis of multilocus fingerprinting data sets containing missing data. Mol. Ecol. Notes, 6, 569-572.
  9. Sugden, D., & Clapperton, C. (1977). The maximum ice extent on island groups in the Scotia Sea Antarctica. Quarternary Research, 7(2), 268-282.
  10. Swofford, D. (2002). PAUP*: Phylogenetic analysis using parsimony (*and other methods). Version 4. Champaign, Illinois: National Illinois History Survey.
  11. Torres-Melado, G., Jana, R., & Casanova-Katny, M. (2011). Antarctic hairgrass expansion in the South Shetland archipelago and Antarctic Peninsula revisited. Polar Biol., 34, 1679-1688.
  12. Vera, M.L. (2011). Colonization and demographic and demographic structure of Deschampsia antarctica and Colobanthus quitensis along an altitudinal gradient on Livingston Island, South Shetland Islands, Antarctica. Polar Res., 30, 7146.
  13. White, T., Bruns, T., Lee, S. et al. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. Innis, D. Gelfand, J. Sninsky, & T. White (Eds.), PCR protocols: a guide to methods and applications. San Diego, Academic Press. pp. 315-322.
  14. Wouw van de, M.J., Dijk van, P.J., Huiskes, A.H.L. (2008). Regional genetic diversity patterns in Antarctic hairgrass (Deschampsia antarctica Desv.). Journal of Biogeography, 35, 365-376.
  15. Smith, R.I.L. (1984). Terrestrial Plant Biology of the Sub-Antarctic and Antarctic. In R.M. Laws (Ed.), Antarctic Ecology, 1, 61-162. London, Academic Press.