Ukrainian Antarctic journal

No 10-11 (2012): Ukrainian Antarctic Journal
Articles

Geographical gradient of genetic diversity of Deschampsіa antarctіca Desv. from the Maritime Antarctic

I. O. Andreev
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
R. A. Volkov
Yuriy Fedkovych Chernivtsi National University, Chernivtsi
I. A. Kozeretska
Taras Shevchenko National University of Kyiv, Kyiv
I.Yu. Parnikoza
Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv
K. V. Spiridonova
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
S. S. Kiryachenko
Taras Shevchenko National University of Kyiv, Kyiv
D. N. Maydanyuk
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv; Luhansk Taras Shevchenko National University, Luhansk
Bio
V. А. Kunakh
Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv
Published December 31, 2012
Keywords
  • Deschampsіa antarctіca,
  • RAPD-analysis,
  • ITS rDNA,
  • Maritime Antarctic

Abstract

In order to study the genetic diversity of D. antarctіca Desv. and phylogenetic relationships between the plants from Antarctica and outside of its borders RAPD-analysis and comparative sequence analysis of rDNA internal transcribed spacers 1 and 2 region (ITS1-2) were conducted. There are 15 samples were studied from two distant regions, namely from King George Island and from islands of Argentine archipelago region. The results of RAPD-analysis indicate the differentiation of the studied populations at the molecular genetic level and the existence of a geographical gradient of genetic diversity of D. antarctіca in Maritime Antarctica with a decrease in this parameter from north to south. Sequence analysis of the ITS1-2 rDNA region revealed the presence of several variants in Antarctic populations that differ by specific mutations from plants growing outside Antarctica.