Ukrainian Antarctic Journal

No 6-7 (2008): Ukrainian Antarctic Journal
Articles

Proteins and lipids of the blood serum and the liver of Antarctic fishes

V. Voitsitsky
Taras Shevchenko National University of Kyiv, Kyiv
S. Khyzhnyk
Taras Shevchenko National University of Kyiv, Kyiv
A. Pavlyk
Taras Shevchenko National University of Kyiv, Kyiv
L. Manilo
National Museum of Natural History, Kyiv
Published December 15, 2008
Keywords
  • adaptation,
  • antarctic fishes,
  • blood serum,
  • proteins,
  • lipids
How to Cite
Voitsitsky, V., Khyzhnyk, S., Pavlyk, A., & Manilo, L. (2008). Proteins and lipids of the blood serum and the liver of Antarctic fishes. Ukrainian Antarctic Journal, (6-7), 104-109. https://doi.org/10.33275/1727-7485.6-7.2008.500

Abstract

It was realized the comparative analysis of protein and lipid contents of the blood serum and the liver of the different species of antarctic fishes. The materials were collected during the 9-th and 11-th Ukrainian antarctic expedition in Argentine islands region. We have defined the presence of the low-molecular protein (glycoprotein) with molecular weight 13kDa in N. coriiceps, P. charcoti, C. aceratus and its absence in T. bernacchii and G. gibberifrons. It was determined that Antarctic fishes is characterized by the features of lipids’ component of the blood serum and liver cells, which may determine the features of its metabolism. Established differences of protein and lipid components of the different species of Antarctic fishes may be interpreted as the species difference, also as the different ways of adaptation to low temperature conditions.

References

  1. Detrich, H.W. (1991). Polymerization of microtubule proteins from Antarctic fish. Biology of Antarctic Fish. Berlin: Springer-Verlag, 248-262.
  2. Fields, P.A., & Somero, G.N. (1998). Hot-spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes. Proceedings of the National Academy of Sciences of the USA, 95, 243-282.
  3. Cocca, E., Ratnayakelecamwasam, M., Parker, S.K., Camardella, L., Ciaramella, M., Di Prisco, G., & Detrich, H.W. (1997). Do the hemoglobinless icefishes have globin genes? Comp. Biochem. Physiol., 118A, 1027-1030.
  4. Sidell, B.D., Vayda, M.E., Small, D.J., Moylan, T.J., Londraville, R.L., Yuan, M. L., Rodnick, K. J., Eppley, Z.A., & Costello, L. (1997). Vaiable expression of myoglobin among the hemoglobinless Antarctic icefishes. Proceedings of the National Academy of Sciences of the USA, 94, 3420-3424.
  5. Somero, G.N., Fields, P.A., Hofmann, G.E., Weinstein, R.B., & Kawall, H. (1998). Cold adaptation and stenothermy in Antarctic notothenioid fishes: what has been gained and what has been lost? Fishes of Antarctica. A Biological Overview. Italia, Springer-Verlag. P. 97-109.
  6. De Vries, A.L. (1988). The role of antifreeze glycopeptides and peptides in the freezing avoidance of Antarctic fishes. Comp. Biochem. Physiol., 90B(3), 611-621.
  7. Isaienko, V. M., Voitsitskyi, V. M., Babeniuk, Yu. D., Khyzhniak, S. V., Ilin, V. M., Oliinyk, S. A. (2005). Ekolohichna biokhimiia. [Eco-friendly biochemistry]. Kyiv, Knyzhkove vyd-vo NAU.
  8. Proxorova,M. [Ed.]. (1982). Metody` bioximicheskix issledovanij. [Biochemical research methods]. L.: Nauka.
  9. Greenberg, C.S., & Craddock, P.R. (1982). Rapid single-step membrane protein assay. Clin. Chem., 28(7), 1725-1726.
  10. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(259), 680-685.
  11. Kucherenko, M. Ie., Babeniuk, Yu. D., Voitsitskyi, V. M. (2001). Suchasn i m et od y biokhimichn y kh d oslidzhen. [Modern methods of biochemical research]. Kyiv, Fitosotsiotsentr.
  12. Voitsitskyi, V., Khyzhniak, S., Manylo, O., Pavlovych, A. (2006). Biokhimichni pokaznyky syrovatky krovi ryb Antarktyky [Biochemical parameters of Antarctic fish serum]. Visnyk Kyivskoho natsionalnoho universytetu imeni Tarasa Shevchenka. Biology, 47–48, 67–68.
  13. Van der Oost, R., Beyer, J., & Vermeulen, N.P.E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13, 57-149.
  14. Kucherenko, M. Ie., Babeniuk, Yu. D., Vasyliev, O. M. at al. (2002). Biokhimiia [Biochemistry]. Kyiv, VPTs «Kyivskyi universytet».
  15. Xochachka, P., Somero, Dzh. (1977). Strategiya bioximicheskoj adaptacii. [Biochemical adaptation strategy]. Moscow, Nauka.